4zr1

X-ray diffraction
2.6Å resolution

Hydroxylase domain of scs7p

Released:

Function and Biology Details

Reactions catalysed:
A phytoceramide + 2 ferrocytochrome b5 + O(2) + 2 H(+) = a (2'R)-2'-hydroxyphytoceramide + 2 ferricytochrome b5 + H(2)O
A dihydroceramide + 2 ferrocytochrome b5 + O(2) + 2 H(+) = a (2'R)-2'-hydroxydihydroceramide + 2 ferricytochrome b5 + H(2)O
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-169915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ceramide very long chain fatty acid hydroxylase SCS7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 35.04 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03529 (Residues: 96-384; Coverage: 75%)
Gene names: FAH1, SCS7, YM8156.14C, YMR272C
Sequence domains: Fatty acid hydroxylase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: I213
Unit cell:
a: 202.762Å b: 202.762Å c: 202.762Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.239
Expression system: Saccharomyces cerevisiae