› 4zr2

X-ray diffraction
1.8Å resolution

Crystal Structure of the Domain-Swapped Dimer K40L:Q108K:Y60W mutant of Human Cellular Retinol Binding Protein II

Released:
DOI: 10.2210/pdb4zr2/pdb
PDB entry 4zr2 coloured by chain, front view.
PDB entry 4zr2 coloured by chain, side view.
PDB entry 4zr2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Domain-Swapped Dimers of Intracellular Lipid-Binding Proteins: Evidence for Ordered Folding Intermediates.
Assar Z, Nossoni Z, Wang W, Santos EM, Kramer K, McCornack C, Vasileiou C, Borhan B, Geiger JH
Structure 24 1590-8 (2016)
PMID: 27524203

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50120 (Residues: 2-134; Coverage: 99%)
Gene names: CRBP2, RBP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand RET 1 x RET
Ligand ACT 5 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21212
Unit cell:
a: 62.768Å b: 109.225Å c: 36.389Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.176 0.218
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
4 other articles

1 mention without citation

Domain-Swapped Dimers of Intracellular Lipid-Binding Proteins: Evidence for Ordered Folding Intermediates.
Assar et al. (2016)