› 4zre

X-ray diffraction
2Å resolution

Crystal structure of SMG1 F278D mutant

Released:
DOI: 10.2210/pdb4zre/pdb
PDB entry 4zre coloured by chain, front view.
PDB entry 4zre coloured by chain, side view.
PDB entry 4zre coloured by chain, top view.
Source organism: Malassezia globosa CBS 7966
Primary publication:
Structure of product-bound SMG1 lipase: active site gating implications.
Guo S, Xu J, Pavlidis IV, Lan D, Bornscheuer UT, Liu J, Wang Y
FEBS J 282 4538-47 (2015)
PMID: 26365206

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Secreted mono- and diacylglycerol lipase LIP1 Chain: A
Molecule details ›
Chain: A
Length: 285 amino acids
Theoretical weight: 31.74 KDa
Source organism: Malassezia globosa CBS 7966
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A8PUY1 (Residues: 20-304; Coverage: 100%)
Gene names: LIP1, MGL_0797, SMG1
Sequence domains: Lipase (class 3)
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: P21
Unit cell:
a: 39.74Å b: 73.239Å c: 44.919Å
α: 90° β: 108.51° γ: 90°
R-values:
R R work R free
0.157 0.157 0.205
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
4 other articles

1 mention without citation

Structure of product-bound SMG1 lipase: active site gating implications.
Guo et al. (2015)