› 4zrl

X-ray diffraction
2.28Å resolution

Structure of the non canonical Poly(A) polymerase complex GLD-2 - GLD-3

Released:
DOI: 10.2210/pdb4zrl/pdb
PDB entry 4zrl coloured by chain, front view.
PDB entry 4zrl coloured by chain, side view.
PDB entry 4zrl coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Structural basis for the activation of the C. elegans noncanonical cytoplasmic poly(A)-polymerase GLD-2 by GLD-3.
Nakel K, Bonneau F, Eckmann CR, Conti E
Proc Natl Acad Sci U S A 112 8614-9 (2015)
PMID: 26124149

Function and Biology Details

Reaction catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127569 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Poly(A) RNA polymerase gld-2 Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 41.96 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O17087 (Residues: 527-923; Coverage: 33%)
Gene names: ZC308.1, gld-2
Sequence domains: Cid1 family poly A polymerase
Defective in germ line development protein 3 Chain: B
Molecule details ›
Chain: B
Length: 77 amino acids
Theoretical weight: 9 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q95ZK7 (Residues: 13-88; Coverage: 8%)
Gene names: T07F8.3, gld-3

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 50.655Å b: 89.367Å c: 94.666Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.23
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Functions and mechanisms of RNA tailing by metazoan terminal nucleotidyltransferases.
Liudkovska et al. (2021)
3 more

3 mentions without citation

Structural basis for the activation of the C. elegans noncanonical cytoplasmic poly(A)-polymerase GLD-2 by GLD-3.
Nakel et al. (2015)
2 more