› 4zrs

X-ray diffraction
2Å resolution

Crystal structure of a cloned feruloyl esterase from a soil metagenomic library

Released:
DOI: 10.2210/pdb4zrs/pdb
PDB entry 4zrs coloured by chain, front view.
PDB entry 4zrs coloured by chain, side view.
PDB entry 4zrs coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Enhancing the Thermostability of Feruloyl Esterase EstF27 by Directed Evolution and the Underlying Structural Basis.
Cao LC, Chen R, Xie W, Liu YH
J Agric Food Chem 63 8225-33 (2015)
PMID: 26329893

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123059 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BD-FAE-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 33.45 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E7DJY5 (Residues: 1-290; Coverage: 100%)
Sequence domains: BD-FAE

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand SEB 2 x SEB

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 52.156Å b: 101.79Å c: 69.822Å
α: 90° β: 110.62° γ: 90°
R-values:
R R work R free
0.152 0.15 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Diversity of fungal feruloyl esterases: updated phylogenetic classification, properties, and industrial applications.
Dilokpimol et al. (2016)
1 more

4 mentions without citation

Metagenomic mining for thermostable esterolytic enzymes uncovers a new family of bacterial esterases.
Zarafeta et al. (2016)
3 more