› 4zrv

X-ray diffraction
2.1Å resolution

Structure of cow mincle CRD complexed with trehalose mono butyrate

Released:
DOI: 10.2210/pdb4zrv/pdb
PDB entry 4zrv coloured by chain, front view.
PDB entry 4zrv coloured by chain, side view.
PDB entry 4zrv coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Binding Sites for Acylated Trehalose Analogs of Glycolipid Ligands on an Extended Carbohydrate Recognition Domain of the Macrophage Receptor Mincle.
Feinberg H, Rambaruth ND, Jégouzo SA, Jacobsen KM, Djurhuus R, Poulsen TB, Weis WI, Taylor ME, Drickamer K
J Biol Chem 291 21222-21233 (2016)
PMID: 27542410

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-122748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
C-type lectin domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 134 amino acids
Theoretical weight: 15.74 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: E1BHM0 (Residues: 79-211; Coverage: 63%)
Gene name: CLEC4E
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, U8V
Ligand GLC 3 x GLC
Ligand U8V 3 x U8V
2 bound ligands:
Ligand CA 9 x CA
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 66.464Å b: 46.676Å c: 77.92Å
α: 90° β: 101.16° γ: 90°
R-values:
R R work R free
0.166 0.163 0.208
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Contact, Collaboration, and Conflict: Signal Integration of Syk-Coupled C-Type Lectin Receptors.
Ostrop et al. (2017)
11 more

8 mentions without citation

Medicinal chemistry of the myeloid C-type lectin receptors Mincle, Langerin, and DC-SIGN.
Cramer J. (2021)
7 more