› 4zs2

X-ray diffraction
2.16Å resolution

Structural complex of FTO/fluorescein

Released:
DOI: 10.2210/pdb4zs2/pdb
PDB entry 4zs2 coloured by chain, front view.
PDB entry 4zs2 coloured by chain, side view.
PDB entry 4zs2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Fluorescein Derivatives as Bifunctional Molecules for the Simultaneous Inhibiting and Labeling of FTO Protein.
Wang T, Hong T, Huang Y, Su H, Wu F, Chen Y, Wei L, Huang W, Hua X, Xia Y, Xu J, Gan J, Yuan B, Feng Y, Zhang X, Yang CG, Zhou X
J Am Chem Soc 137 13736-9 (2015)
PMID: 26457839

Function and Biology Details

Reaction catalysed: 
N(6)-methyladenosine in mRNA + 2-oxoglutarate + O(2) = adenosine in mRNA + formaldehyde + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-ketoglutarate-dependent dioxygenase FTO Chain: A
Molecule details ›
Chain: A
Length: 478 amino acids
Theoretical weight: 54.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9C0B1 (Residues: 32-505; Coverage: 94%)
Gene names: FTO, KIAA1752
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand AKG 1 x AKG
Ligand FLU 1 x FLU
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: R3
Unit cell:
a: 142.66Å b: 142.66Å c: 83.218Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.198 0.229
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Reversible N6-methyladenosine of RNA: The regulatory mechanisms on gene expression and implications in physiology and pathology.
Fang et al. (2020)
24 more

7 mentions without citation

Recent Advances of m6A Demethylases Inhibitors and Their Biological Functions in Human Diseases.
You et al. (2022)
6 more