› 4zs9

X-ray diffraction
1.37Å resolution

Raffinose and panose binding protein from Bifidobacterium animalis subsp. lactis Bl-04, bound with raffinose

Released:
DOI: 10.2210/pdb4zs9/pdb
PDB entry 4zs9 coloured by chain, front view.
PDB entry 4zs9 coloured by chain, side view.
PDB entry 4zs9 coloured by chain, top view.
Source organism: Bifidobacterium animalis subsp. lactis Bl-04
Primary publication:
An ATP Binding Cassette Transporter Mediates the Uptake of α-(1,6)-Linked Dietary Oligosaccharides in Bifidobacterium and Correlates with Competitive Growth on These Substrates.
Ejby M, Fredslund F, Andersen JM, Vujičić Žagar A, Henriksen JR, Andersen TL, Svensson B, Slotboom DJ, Abou Hachem M
J Biol Chem 291 20220-31 (2016)
PMID: 27502277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103216 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sugar binding protein of ABC transporter system Chains: A, B
Molecule details ›
Chains: A, B
Length: 413 amino acids
Theoretical weight: 46.1 KDa
Source organism: Bifidobacterium animalis subsp. lactis Bl-04
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1A9TA69 (Residues: 2-393; Coverage: 100%)
Gene name: Balac_1599
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: FRU, GLC, GLA
Carbohydrate polymer
2 bound ligands:
Ligand MG 15 x MG
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 55.54Å b: 90.82Å c: 146.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.132 0.167
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

The Critical Roles of Polysaccharides in Gut Microbial Ecology and Physiology.
Porter et al. (2017)
5 more

2 mentions without citation

Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes.
Fushinobu et al. (2021)
1 more