› 4zt0

X-ray diffraction
2.9Å resolution

Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution

Released:
DOI: 10.2210/pdb4zt0/pdb
PDB entry 4zt0 coloured by chain, front view.
PDB entry 4zt0 coloured by chain, side view.
PDB entry 4zt0 coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
STRUCTURAL BIOLOGY. A Cas9-guide RNA complex preorganized for target DNA recognition.
Jiang F, Zhou K, Ma L, Gressel S, Doudna JA
Science 348 1477-81 (2015)
PMID: 26113724

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189432 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 1369 amino acids
Theoretical weight: 159.8 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 2-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
single-guide RNA Chains: B, D
Molecule details ›
Chains: B, D
Length: 85 nucleotides
Theoretical weight: 27.5 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 44 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 103.238Å b: 143.002Å c: 294.917Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.238 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

79 review citations

Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors.
Anzalone et al. (2020)
78 more

39 mentions without citation

The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit.
Murugan et al. (2017)
38 more