› 4ztx

X-ray diffraction
2.1Å resolution

Neurospora crassa cobalamin-independent methionine synthase complexed with Zn2+

Released:
DOI: 10.2210/pdb4ztx/pdb
PDB entry 4ztx coloured by chain, front view.
PDB entry 4ztx coloured by chain, side view.
PDB entry 4ztx coloured by chain, top view.
Source organism: Neurospora crassa
Primary publication:
Fungal cobalamin-independent methionine synthase: Insights from the model organism, Neurospora crassa.
Wheatley RW, Ng KKS, Kapoor M
Arch Biochem Biophys 590 125-137 (2016)
PMID: 26657067

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 769 amino acids
Theoretical weight: 86.43 KDa
Source organism: Neurospora crassa
UniProt:
  • Canonical: Q8X1E4 (Residues: 1-769; Coverage: 100%)
Gene name: met-8
Sequence domains:
Structure domains: TIM Barrel

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand NO3 1 x NO3
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 78.254Å b: 94.63Å c: 114.907Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.163 0.217

Quick links

Citations

4 citation in other articles

Systems impact of zinc chelation by the epipolythiodioxopiperazine dithiol gliotoxin in Aspergillus fumigatus: a new direction in natural product functionality.
Saleh et al. (2018)
3 more

1 mention without citation

Identification and characterization of a bacterial core methionine synthase.
Deobald et al. (2020)