› 4zus

X-ray diffraction
2.6Å resolution

Crystal structure of Equine MHC I(Eqca-N*00602) in complexed with equine infectious anaemia virus (EIAV) derived peptide REV-QW11

Released:
DOI: 10.2210/pdb4zus/pdb
PDB entry 4zus coloured by chain, front view.
PDB entry 4zus coloured by chain, side view.
PDB entry 4zus coloured by chain, top view.
Source organisms:
Primary publication:
Structural Illumination of Equine MHC Class I Molecules Highlights Unconventional Epitope Presentation Manner That Is Evolved in Equine Leukocyte Antigen Alleles.
Yao S, Liu J, Qi J, Chen R, Zhang N, Liu Y, Wang J, Wu Y, Gao GF, Xia C
J Immunol 196 1943-54 (2016)
PMID: 26764037

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135074 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.6 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q860N6 (Residues: 22-295; Coverage: 82%)
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Protein Rev Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.4 KDa
Source organism: Equine infectious anemia virus
Expression system: Not provided
UniProt:
  • Canonical: P11305 (Residues: 87-97; Coverage: 7%)
Gene name: rev

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 68.97Å b: 68.97Å c: 205.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

New vistas unfold: Chicken MHC molecules reveal unexpected ways to present peptides to the immune system.
Halabi et al. (2022)
12 other articles

1 mention without citation

The Mechanism of β2m Molecule-Induced Changes in the Peptide Presentation Profile in a Bony Fish.
Li et al. (2020)