› 4zvg

X-ray diffraction
2.2Å resolution

Crystal structure of GGDEF domain of the E. coli DosC - form III

Released:
DOI: 10.2210/pdb4zvg/pdb
Model geometry
Fit model/data
PDB entry 4zvg coloured by chain, front view.
PDB entry 4zvg coloured by chain, side view.
PDB entry 4zvg coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural analysis of an oxygen-regulated diguanylate cyclase.
Tarnawski M, Barends TR, Schlichting I
Acta Crystallogr D Biol Crystallogr 71 2158-77 (2015)
PMID: 26527135

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141975 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diguanylate cyclase DosC Chains: A, B
Molecule details ›
Chains: A, B
Length: 172 amino acids
Theoretical weight: 19.9 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA89 (Residues: 297-460; Coverage: 36%)
Gene names: JW5241, b1490, dgcO, dosC, yddV
Sequence domains: Diguanylate cyclase, GGDEF domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 53.78Å b: 58.93Å c: 113.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.252
Expression system: Escherichia coli

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Citations

4 review citations

C-di-GMP Synthesis: Structural Aspects of Evolution, Catalysis and Regulation.
Schirmer T. (2016)
3 more

1 mention without citation

Mechanism and Role of Globin-Coupled Sensor Signalling.
Walker et al. (2017)