› 4zwn

X-ray diffraction
2.49Å resolution

Crystal Structure of a Soluble Variant of the Monoglyceride Lipase from Saccharomyces Cerevisiae

Released:
DOI: 10.2210/pdb4zwn/pdb
PDB entry 4zwn coloured by chain, front view.
PDB entry 4zwn coloured by chain, side view.
PDB entry 4zwn coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structure of the Saccharomyces cerevisiae monoglyceride lipase Yju3p.
Aschauer P, Rengachari S, Lichtenegger J, Schittmayer M, Das KM, Mayer N, Breinbauer R, Birner-Gruenberger R, Gruber CC, Zimmermann R, Gruber K, Oberer M
Biochim Biophys Acta 1861 462-70 (2016)
PMID: 26869448

Function and Biology Details

Reaction catalysed: 
Hydrolyzes glycerol monoesters of long-chain fatty acids
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monoglyceride lipase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 334 amino acids
Theoretical weight: 38.04 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28321 (Residues: 2-313; Coverage: 100%)
Gene names: YJU3, YKL094W, YKL441
Sequence domains: Serine aminopeptidase, S33
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand NO3 1 x NO3
Ligand SO4 2 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 77.196Å b: 108.566Å c: 167.667Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 citation in other articles

Hypoxia-inducible lipid droplet-associated protein inhibits adipose triglyceride lipase.
Padmanabha Das et al. (2018)
9 more

2 mentions without citation

Structure of the Catalytic Domain of the Class I Polyhydroxybutyrate Synthase from Cupriavidus necator.
Wittenborn et al. (2016)
1 more