› 4zyk

X-ray diffraction
2Å resolution

Crystal Structure of Quaternary-Specific RSV-Neutralizing Human Antibody AM14

Released:
DOI: 10.2210/pdb4zyk/pdb
PDB entry 4zyk coloured by chain, front view.
PDB entry 4zyk coloured by chain, side view.
PDB entry 4zyk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Characterization of a Prefusion-Specific Antibody That Recognizes a Quaternary, Cleavage-Dependent Epitope on the RSV Fusion Glycoprotein.
Gilman MS, Moin SM, Mas V, Chen M, Patel NK, Kramer K, Zhu Q, Kabeche SC, Kumar A, Palomo C, Beaumont T, Baxa U, Ulbrandt ND, Melero JA, Graham BS, McLellan JS
PLoS Pathog 11 e1005035 (2015)
PMID: 26161532

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230436 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AM14 Fab heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 227 amino acids
Theoretical weight: 24.37 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
AM14 light chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 215 amino acids
Theoretical weight: 23.62 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand EDO 5 x EDO
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 69.68Å b: 95.88Å c: 145.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.226
Expression system: Homo sapiens

Quick links

Citations

15 review citations

Heparan Sulfate Proteoglycans and Viral Attachment: True Receptors or Adaptation Bias?
Cagno et al. (2019)
14 more

5 mentions without citation

An HIV-1 antibody from an elite neutralizer implicates the fusion peptide as a site of vulnerability.
van Gils et al. (2016)
4 more