› 4zyo

X-ray diffraction
3.25Å resolution

Crystal Structure of Human Integral Membrane Stearoyl-CoA Desaturase with Substrate

Released:
DOI: 10.2210/pdb4zyo/pdb
PDB entry 4zyo coloured by chain, front view.
PDB entry 4zyo coloured by chain, side view.
PDB entry 4zyo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human stearoyl-coenzyme A desaturase in complex with substrate.
Wang H, Klein MG, Zou H, Lane W, Snell G, Levin I, Li K, Sang BC
Nat Struct Mol Biol 22 581-5 (2015)
PMID: 26098317

Function and Biology Details

Reaction catalysed: 
Stearoyl-CoA + 2 ferrocytochrome b5 + O(2) + 2 H(+) = oleoyl-CoA + 2 ferricytochrome b5 + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126393 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stearoyl-CoA desaturase Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 36.8 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O00767 (Residues: 45-359; Coverage: 88%)
Gene names: FADS5, SCD, SCD1, SCDOS

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand ST9 1 x ST9
Ligand LMT 1 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3121
Unit cell:
a: 134.108Å b: 134.108Å c: 112.561Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.241 0.264
Expression system: Spodoptera frugiperda

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Citations

18 review citations

Targeting cancer metabolism in the era of precision oncology.
Stine et al. (2022)
17 more

22 mentions without citation

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Nile et al. (2019)
21 more