› 5a0w

X-ray diffraction
2.2Å resolution

THE CRYSTAL STRUCTURE OF I-DMOI E117A IN COMPLEX WITH ITS TARGET DNA AND IN THE PRESENCE OF 2MM MN

Released:
DOI: 10.2210/pdb5a0w/pdb
PDB entry 5a0w coloured by chain, front view.
PDB entry 5a0w coloured by chain, side view.
PDB entry 5a0w coloured by chain, top view.
Source organisms:
Entry authors: Molina R, Besker N, Prieto J, Montoya G, D'Abramo M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118389 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease I-DmoI Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 199 amino acids
Theoretical weight: 23.21 KDa
Source organism: Desulfurococcus mucosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21505 (Residues: 2-188; Coverage: 96%)
Sequence domains: LAGLIDADG-like domain
Structure domains: Homing endonucleases
25MER Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 25 nucleotides
Theoretical weight: 7.71 KDa
Source organism: synthetic construct
Expression system: Not provided
25MER Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 25 nucleotides
Theoretical weight: 7.65 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand CL 7 x CL
Ligand ACT 5 x ACT
Ligand MN 6 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 106.928Å b: 69.77Å c: 106.985Å
α: 90° β: 120.06° γ: 90°
R-values:
R R work R free
0.172 0.171 0.208
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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