› 5a10

X-ray diffraction
1.42Å resolution

The crystal structure of Ta-TFP, a thiocyanate-forming protein involved in glucosinolate breakdown (space group C2)

Released:
DOI: 10.2210/pdb5a10/pdb
PDB entry 5a10 coloured by chain, front view.
PDB entry 5a10 coloured by chain, side view.
PDB entry 5a10 coloured by chain, top view.
Source organism: Thlaspi arvense
Primary publication:
The crystal structure of the thiocyanate-forming protein from Thlaspi arvense, a kelch protein involved in glucosinolate breakdown.
Gumz F, Krausze J, Eisenschmidt D, Backenköhler A, Barleben L, Brandt W, Wittstock U
Plant Mol Biol 89 67-81 (2015)
PMID: 26260516

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-(sulfonatooxy)prop-2-enimidothioate sulfolyase Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 39.75 KDa
Source organism: Thlaspi arvense
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G1FNI6 (Residues: 1-348; Coverage: 100%)
Gene name: TFP
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: C2
Unit cell:
a: 119.39Å b: 48.96Å c: 68.27Å
α: 90° β: 109.71° γ: 90°
R-values:
R R work R free
0.158 0.157 0.176
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways.
Qin et al. (2023)
2 other articles

10 mentions without citation

A Systematic Review of In vitro and In vivo Activities of Anti-Toxoplasma Drugs and Compounds (2006-2016).
Montazeri et al. (2017)
9 more