› 5a1s

X-ray diffraction
2.5Å resolution

Crystal structure of the sodium-dependent citrate symporter SeCitS form Salmonella enterica.

Released:
DOI: 10.2210/pdb5a1s/pdb
PDB entry 5a1s coloured by chain, front view.
PDB entry 5a1s coloured by chain, side view.
PDB entry 5a1s coloured by chain, top view.
Source organism: Salmonella enterica
Primary publication:
Mechanism of Na(+)-dependent citrate transport from the structure of an asymmetrical CitS dimer.
Wöhlert D, Grötzinger MJ, Kühlbrandt W, Yildiz Ö
Elife 4 e09375 (2015)
PMID: 26636752

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165421 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CITRATE-SODIUM SYMPORTER Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 448 amino acids
Theoretical weight: 47.91 KDa
Source organism: Salmonella enterica
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

7 bound ligands:
Ligand NA 7 x NA
Ligand FLC 7 x FLC
Ligand BOG 5 x BOG
Ligand UND 2 x UND
Ligand CL 3 x CL
Ligand PTY 1 x PTY
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 86.382Å b: 89.935Å c: 91.843Å
α: 90.44° β: 113.79° γ: 99.55°
R-values:
R R work R free
0.21 0.208 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
5 more

9 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
8 more