› 5a21

Electron Microscopy
7.2Å resolution

Structure of bacteriophage SPP1 head-to-tail interface without DNA and tape measure protein

Released:
DOI: 10.2210/pdb5a21/pdb
PDB entry 5a21 coloured by chain, front view.
PDB entry 5a21 coloured by chain, side view.
PDB entry 5a21 coloured by chain, top view.
Source organism: Bacillus phage SPP1
Primary publication:
Structural rearrangements in the phage head-to-tail interface during assembly and infection.
Chaban Y, Lurz R, Brasilès S, Cornilleau C, Karreman M, Zinn-Justin S, Tavares P, Orlova EV
Proc Natl Acad Sci U S A 112 7009-14 (2015)
PMID: 25991862
Related structures: EMD-2994

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-129234 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Portal protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 503 amino acids
Theoretical weight: 57.41 KDa
Source organism: Bacillus phage SPP1
UniProt:
  • Canonical: P54309 (Residues: 1-503; Coverage: 100%)
Gene names: 6, siz
Sequence domains: Phage portal protein, SPP1 Gp6-like
Head completion protein gp15 Chains: C, D
Molecule details ›
Chains: C, D
Length: 102 amino acids
Theoretical weight: 11.63 KDa
Source organism: Bacillus phage SPP1
UniProt:
  • Canonical: Q38584 (Residues: 1-102; Coverage: 100%)
Gene name: 15
Sequence domains: Phage gp6-like head-tail connector protein
Head completion protein gp16 Chains: E, F
Molecule details ›
Chains: E, F
Length: 109 amino acids
Theoretical weight: 12.62 KDa
Source organism: Bacillus phage SPP1
UniProt:
  • Canonical: O48446 (Residues: 1-109; Coverage: 100%)
Gene name: 16
Sequence domains: Phage head-tail joining protein
Tail completion protein gp17 Chain: G
Molecule details ›
Chain: G
Length: 134 amino acids
Theoretical weight: 15.03 KDa
Source organism: Bacillus phage SPP1
UniProt:
  • Canonical: O48448 (Residues: 1-134; Coverage: 100%)
Sequence domains: Protein of unknown function (DUF3168)
Tail tube protein gp17.1* Chain: H
Molecule details ›
Chain: H
Length: 177 amino acids
Theoretical weight: 19.17 KDa
Source organism: Bacillus phage SPP1
UniProt:
  • Canonical: O48449 (Residues: 1-177; Coverage: 67%)
Gene name: 17.1
Sequence domains: Phage tail tube protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.2Å
Relevant EMDB volumes: EMD-2994

Quick links

Citations

7 review citations

Portal Protein: The Orchestrator of Capsid Assembly for the dsDNA Tailed Bacteriophages and Herpesviruses.
Dedeo et al. (2019)
6 more

9 mentions without citation

The three-part model for coding causes and mechanisms of healthcare-related adverse events.
Southern et al. (2022)
8 more