› 5a2z

X-ray diffraction
2.45Å resolution

Crystal structure of mtPAP in complex with GTP

Released:
DOI: 10.2210/pdb5a2z/pdb
PDB entry 5a2z coloured by chain, front view.
PDB entry 5a2z coloured by chain, side view.
PDB entry 5a2z coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structure of mitochondrial poly(A) RNA polymerase reveals the structural basis for dimerization, ATP selectivity and the SPAX4 disease phenotype.
Lapkouski M, Hällberg BM
Nucleic Acids Res 43 9065-75 (2015)
PMID: 26319014

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RL domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 555 amino acids
Theoretical weight: 62.95 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: F1NBW0 (Residues: 37-568; Coverage: 94%)
Gene name: MTPAP
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.3
Spacegroup: P212121
Unit cell:
a: 59.746Å b: 94.364Å c: 192.014Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.26 0.295
Expression system: Escherichia coli

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Citations

5 review citations

Mitochondrial Dysfunction in Parkinson's Disease: Focus on Mitochondrial DNA.
Buneeva et al. (2020)
4 more

1 mention without citation

Structure of mitochondrial poly(A) RNA polymerase reveals the structural basis for dimerization, ATP selectivity and the SPAX4 disease phenotype.
Lapkouski et al. (2015)