› 5a39

X-ray diffraction
2.8Å resolution

Structure of Rad14 in complex with cisplatin containing DNA

Released:
DOI: 10.2210/pdb5a39/pdb
PDB entry 5a39 coloured by chain, front view.
PDB entry 5a39 coloured by chain, side view.
PDB entry 5a39 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural insights into the recognition of cisplatin and AAF-dG lesion by Rad14 (XPA).
Koch SC, Kuper J, Gasteiger KL, Simon N, Strasser R, Eisen D, Geiger S, Schneider S, Kisker C, Carell T
Proc Natl Acad Sci U S A 112 8272-7 (2015)
PMID: 26100901

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118390 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA repair protein RAD14 Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 13.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P28519 (Residues: 188-302; Coverage: 31%)
Gene names: RAD14, YM8325.02C, YMR201C
Sequence domains: XPA protein C-terminus
DNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 15 nucleotides
Theoretical weight: 4.52 KDa
Source organism: Saccharomyces cerevisiae
5'-D(*DTP*GP*AP*TP*GP*AP*CP*CP*GP*TP*AP*GP*AP)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 13 nucleotides
Theoretical weight: 4.02 KDa
Source organism: Saccharomyces cerevisiae
5'-D(*DGP*AP*TP*GP*AP*CP*CP*GP*TP*AP*GP*AP*GP)-3' Chains: G, H
Molecule details ›
Chains: G, H
Length: 13 nucleotides
Theoretical weight: 4.04 KDa
Source organism: Saccharomyces cerevisiae

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CPT 4 x CPT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P41
Unit cell:
a: 54.421Å b: 54.421Å c: 130.773Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.256
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Role of the XPA protein in the NER pathway: A perspective on the function of structural disorder in macromolecular assembly.
Fadda E. (2016)
3 more

5 mentions without citation

XPA: A key scaffold for human nucleotide excision repair.
Sugitani et al. (2016)
4 more