› 5a40

X-ray diffraction
3.6Å resolution

Crystal structure of a dual topology fluoride ion channel.

Released:
DOI: 10.2210/pdb5a40/pdb
PDB entry 5a40 coloured by chain, front view.
PDB entry 5a40 coloured by chain, side view.
PDB entry 5a40 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of a double-barrelled fluoride ion channel.
Stockbridge RB, Kolmakova-Partensky L, Shane T, Koide A, Koide S, Miller C, Newstead S
Nature 525 548-51 (2015)
PMID: 26344196

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165423 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fluoride-specific ion channel FluC Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 13.29 KDa
Source organism: Bordetella pertussis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7VYU0 (Residues: 1-128; Coverage: 100%)
Gene names: BP1217, crcB, fluC
Sequence domains: CrcB-like protein, Camphor Resistance (CrcB)
MONOBODIES Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 90 amino acids
Theoretical weight: 10.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand HG 4 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21212
Unit cell:
a: 146.79Å b: 183.7Å c: 72.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.235 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Monobodies and other synthetic binding proteins for expanding protein science.
Sha et al. (2017)
12 more

3 mentions without citation

Crystal structures of a double-barrelled fluoride ion channel.
Stockbridge et al. (2015)
2 more