› 5a4r

X-ray diffraction
2.25Å resolution

Crystal structure of a vitamin B12 trafficking protein

Released:
DOI: 10.2210/pdb5a4r/pdb
PDB entry 5a4r coloured by chain, front view.
PDB entry 5a4r coloured by chain, side view.
PDB entry 5a4r coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural Insights into the MMACHC-MMADHC Protein Complex Involved in Vitamin B12 Trafficking.
Froese DS, Kopec J, Fitzpatrick F, Schuller M, McCorvie TJ, Chalk R, Plessl T, Fettelschoss V, Fowler B, Baumgartner MR, Yue WW
J Biol Chem 290 29167-77 (2015)
PMID: 26483544

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189349 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cobalamin trafficking protein CblD Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 19.22 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99LS1 (Residues: 129-296; Coverage: 57%)
Gene name: Mmadhc
Sequence domains: Methylmalonic aciduria and homocystinuria type D protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 74.21Å b: 89.53Å c: 64.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Mass Spectrometry-Based Metabolomic and Proteomic Strategies in Organic Acidemias.
Imperlini et al. (2016)
3 more

2 mentions without citation

From structural biology to designing therapy for inborn errors of metabolism.
Yue WW. (2016)
1 more