› 5a52

X-ray diffraction
1.65Å resolution

The crystal structure of Arabidopsis thaliana CAR1 in complex with one calcium ion

Released:
DOI: 10.2210/pdb5a52/pdb
PDB entry 5a52 coloured by chain, front view.
PDB entry 5a52 coloured by chain, side view.
PDB entry 5a52 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling.
Diaz M, Sanchez-Barrena MJ, Gonzalez-Rubio JM, Rodriguez L, Fernandez D, Antoni R, Yunta C, Belda-Palazon B, Gonzalez-Guzman M, Peirats-Llobet M, Menendez M, Boskovic J, Marquez JA, Rodriguez PL, Albert A
Proc Natl Acad Sci U S A 113 E396-405 (2016)
PMID: 26719420

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein C2-DOMAIN ABA-RELATED 1 Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 18.87 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FHP6 (Residues: 1-168; Coverage: 100%)
Gene names: At5g37740, CAR1, K12B20.22
Sequence domains: C2 domain

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 39.6Å b: 170.3Å c: 53.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.243
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Advances and current challenges in calcium signaling.
Kudla et al. (2018)
1 more

2 mentions without citation

Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling.
Diaz et al. (2016)
1 more