› 5a6v

X-ray diffraction
2.28Å resolution

Open and closed conformations and protonation states of Candida antarctica Lipase B: Xenon complex

Released:
DOI: 10.2210/pdb5a6v/pdb
PDB entry 5a6v coloured by chain, front view.
PDB entry 5a6v coloured by chain, side view.
PDB entry 5a6v coloured by chain, top view.
Source organism: Moesziomyces antarcticus
Primary publication:
Open and closed states of Candida antarctica lipase B: protonation and the mechanism of interfacial activation.
Stauch B, Fisher SJ, Cianci M
J Lipid Res 56 2348-58 (2015)
PMID: 26447231

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154387 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lipase B Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 33.04 KDa
Source organism: Moesziomyces antarcticus
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: P41365 (Residues: 26-342; Coverage: 98%)
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand IPA 2 x IPA
Ligand XE 6 x XE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P1
Unit cell:
a: 39.656Å b: 48.89Å c: 71.68Å
α: 87.8° β: 98.07° γ: 108.17°
R-values:
R R work R free
0.142 0.139 0.195
Expression system: Aspergillus oryzae

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Citations

5 review citations

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
4 more

7 mentions without citation

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.
Magalhães et al. (2021)
6 more