› 5a70

X-ray diffraction
1.6Å resolution

Structure of the LecB lectin from Pseudomonas aeruginosa strain PA14 in complex with lewis x tetrasaccharide

Released:
DOI: 10.2210/pdb5a70/pdb
PDB entry 5a70 coloured by chain, front view.
PDB entry 5a70 coloured by chain, side view.
PDB entry 5a70 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Primary publication:
Induction of rare conformation of oligosaccharide by binding to calcium-dependent bacterial lectin: X-ray crystallography and modelling study.
Lepsik M, Sommer R, Kuhaudomlarp S, Lelimousin M, Paci E, Varrot A, Titz A, Imberty A
Eur J Med Chem 177 212-220 (2019)
PMID: 31146126

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Calcium-mediated lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 114 amino acids
Theoretical weight: 11.68 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2ZE85 (Residues: 2-115; Coverage: 99%)
Gene names: PA14_20610, lecB
Sequence domains: Fucose-binding lectin II (PA-IIL)
Structure domains: Calcium-mediated lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand CA 8 x CA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 52.898Å b: 63.228Å c: 63.889Å
α: 90° β: 91.2° γ: 90°
R-values:
R R work R free
0.138 0.136 0.171
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Increasing the Affinity of an O-Antigen Polysaccharide Binding Site in Shigella flexneri Bacteriophage Sf6 Tailspike Protein.
Kunstmann et al. (2020)
1 more

1 mention without citation

Targeting undruggable carbohydrate recognition sites through focused fragment library design.
Shanina et al. (2022)