5a71

X-ray diffraction
0.91Å resolution

Open and closed conformations and protonation states of Candida antarctica Lipase B: atomic resolution native

Released:

Function and Biology Details

Reaction catalysed:
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154387 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lipase B Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 33.04 KDa
Source organism: Moesziomyces antarcticus
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: P41365 (Residues: 26-342; Coverage: 98%)
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P1
Unit cell:
a: 39.67Å b: 48.94Å c: 71.61Å
α: 88.74° β: 97.15° γ: 108.44°
R-values:
R R work R free
0.113 0.112 0.132
Expression system: Aspergillus oryzae