› 5a8c

X-ray diffraction
0.97Å resolution

The ultra high resolution structure of a novel alpha-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405 with bound trimethyl N-Oxide (TRS)

Released:
DOI: 10.2210/pdb5a8c/pdb
PDB entry 5a8c coloured by chain, front view.
PDB entry 5a8c coloured by chain, side view.
PDB entry 5a8c coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Molecular determinants of substrate specificity revealed by the structure of Clostridium thermocellum arabinofuranosidase 43A from glycosyl hydrolase family 43 subfamily 16.
Goyal A, Ahmed S, Sharma K, Gupta V, Bule P, Alves VD, Fontes CM, Najmudin S
Acta Crystallogr D Struct Biol 72 1281-1289 (2016)
PMID: 27917828

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107052 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate binding family 6 Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 36.45 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3DEX4 (Residues: 30-330; Coverage: 44%)
Gene name: Cthe_1271
Sequence domains: Glycosyl hydrolases family 43
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 45.44Å b: 67.7Å c: 51.01Å
α: 90° β: 113.09° γ: 90°
R-values:
R R work R free
0.104 0.103 0.124
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

GH62 arabinofuranosidases: Structure, function and applications.
Wilkens et al. (2017)
3 other articles

3 mentions without citation

Identification and characterization of GH11 xylanase and GH43 xylosidase from the chytridiomycetous fungus, Rhizophlyctis rosea.
Huang et al. (2019)
2 more