5a8l

Electron Microscopy
3.8Å resolution

Human eRF1 and the hCMV nascent peptide in the translation termination complex

Released:
Source organism: Homo sapiens
Primary publication:
Structure of a human translation termination complex.
Nucleic Acids Res 43 8615-26 (2015)
PMID: 26384426
Related structures: EMD-3099

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-215964 (preferred)
Entry contents:
5 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (9 distinct):
Large ribosomal subunit protein uL22 Chain: D
Molecule details ›
Chain: D
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18621 (Residues: 1-184; Coverage: 100%)
Gene name: RPL17
Sequence domains: Ribosomal protein L22p/L17e
Large ribosomal subunit protein uL11 Chain: G
Molecule details ›
Chain: G
Length: 165 amino acids
Theoretical weight: 17.85 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P30050 (Residues: 1-165; Coverage: 100%)
Gene name: RPL12
Sequence domains:
Large ribosomal subunit protein uL4 Chain: H
Molecule details ›
Chain: H
Length: 427 amino acids
Theoretical weight: 47.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P36578 (Residues: 1-427; Coverage: 100%)
Gene names: RPL1, RPL4
Sequence domains:
Eukaryotic peptide chain release factor subunit 1 Chain: Q
Molecule details ›
Chain: Q
Length: 431 amino acids
Theoretical weight: 48.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62495 (Residues: 7-437; Coverage: 99%)
Gene names: ERF1, ETF1, RF1, SUP45L1
Sequence domains:
NASCENT CHAIN Chain: Z
Molecule details ›
Chain: Z
Length: 22 amino acids
Theoretical weight: 2.44 KDa
Source organism: Homo sapiens
28S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 5025 nucleotides
Theoretical weight: 1625.94 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
18S Ribosomal RNA Chain: B
Molecule details ›
Chain: B
Length: 1869 nucleotides
Theoretical weight: 602.78 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
P-SITE TRNA Chain: P
Molecule details ›
Chain: P
Length: 18 nucleotides
Theoretical weight: 5.77 KDa
Messenger RNA Chain: R
Molecule details ›
Chain: R
Length: 9 nucleotides
Theoretical weight: 2.82 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-3099