› 5aaw

X-ray diffraction
3.27Å resolution

Structure of a redesigned cross-reactive antibody to dengue virus with increased in vivo potency

Released:
DOI: 10.2210/pdb5aaw/pdb
PDB entry 5aaw coloured by chain, front view.
PDB entry 5aaw coloured by chain, side view.
PDB entry 5aaw coloured by chain, top view.
Source organisms:
Primary publication:
Structure-Guided Design of an Anti-dengue Antibody Directed to a Non-immunodominant Epitope.
Robinson LN, Tharakaraman K, Rowley KJ, Costa VV, Chan KR, Wong YH, Ong LC, Tan HC, Koch T, Cain D, Kirloskar R, Viswanathan K, Liew CW, Tissire H, Ramakrishnan B, Myette JR, Babcock GJ, Sasisekharan V, Alonso S, Chen J, Lescar J, Shriver Z, Ooi EE, Sasisekharan R
Cell 162 493-504 (2015)
PMID: 26189681

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212421 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SCFV513 Chains: A, B, D, F, H, K
Molecule details ›
Chains: A, B, D, F, H, K
Length: 252 amino acids
Theoretical weight: 27.43 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
DENGUE SEROTYPE 4 ENVELOPE PROTEIN DOMAIN 3 Chains: C, E, G, I, J, L
Molecule details ›
Chains: C, E, G, I, J, L
Length: 115 amino acids
Theoretical weight: 12.69 KDa
Source organism: Dengue virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6YFR6 (Residues: 571-679; Coverage: 13%)
Sequence domains: Flavivirus glycoprotein, immunoglobulin-like domain
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 87.11Å b: 131.67Å c: 176.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.227 0.263
Expression system: Escherichia coli

Quick links

Citations

36 review citations

New insights into the immunopathology and control of dengue virus infection.
Screaton et al. (2015)
35 more

3 mentions without citation

Rational Engineering and Characterization of an mAb that Neutralizes Zika Virus by Targeting a Mutationally Constrained Quaternary Epitope.
Tharakaraman et al. (2018)
2 more