› 5adx

Electron Microscopy
4Å resolution

CryoEM structure of dynactin complex at 4.0 angstrom resolution

Released:
DOI: 10.2210/pdb5adx/pdb
PDB entry 5adx coloured by chain, front view.
PDB entry 5adx coloured by chain, side view.
PDB entry 5adx coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
The structure of the dynactin complex and its interaction with dynein.
Urnavicius L, Zhang K, Diamant AG, Motz C, Schlager MA, Yu M, Patel NA, Robinson CV, Carter AP
Science 347 1441-1446 (2015)
PMID: 25814576
Related structures: EMD-2856

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 27-mer (preferred)
PDBe Complex ID:
PDB-CPX-102281 (preferred)
Entry contents:
17 distinct polypeptide molecules
Macromolecules (17 distinct):
Alpha-centractin Chains: A, B, C, D, E, F, G, I
Molecule details ›
Chains: A, B, C, D, E, F, G, I
Length: 370 amino acids
Theoretical weight: 41.96 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F2Z5G5 (Residues: 7-310; Coverage: 87%)
Gene name: ACTR1A
Sequence domains: Actin
Structure domains:
Actin, cytoplasmic 1 Chain: H
Molecule details ›
Chain: H
Length: 370 amino acids
Theoretical weight: 41.19 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q6QAQ1 (Residues: 6-375; Coverage: 99%)
Gene name: ACTB
Sequence domains: Actin
Structure domains:
Actin-related protein 10 Chain: J
Molecule details ›
Chain: J
Length: 417 amino acids
Theoretical weight: 46.25 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: I3LHK5 (Residues: 1-417; Coverage: 100%)
Gene names: ACTR10, ARP11
Sequence domains: Actin
F-actin-capping protein subunit alpha-1 Chain: K
Molecule details ›
Chain: K
Length: 275 amino acids
Theoretical weight: 31.78 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0PFK5 (Residues: 7-281; Coverage: 96%)
Gene names: CAPZA1, CP alpha1, Capza
Sequence domains: F-actin capping protein alpha subunit
Structure domains:
F-actin-capping protein subunit beta Chain: L
Molecule details ›
Chain: L
Length: 270 amino acids
Theoretical weight: 30.51 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: D2JYW4 (Residues: 2-271; Coverage: 98%)
Gene name: CAPZB
Sequence domains: F-actin capping protein, beta subunit
Structure domains:
DYNACTIN SUBUNIT 2 Chain: M
Molecule details ›
Chain: M
Length: 824 amino acids
Theoretical weight: 70.14 KDa
Source organism: Sus scrofa
DYNACTIN SUBUNIT 2 Chain: N
Molecule details ›
Chain: N
Length: 818 amino acids
Theoretical weight: 69.63 KDa
Source organism: Sus scrofa
DYNACTIN SUBUNIT 3 Chains: O, P
Molecule details ›
Chains: O, P
Length: 88 amino acids
Theoretical weight: 7.51 KDa
Source organism: Sus scrofa
DYNACTIN SUBUNIT 2 Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 91 amino acids
Theoretical weight: 7.76 KDa
Source organism: Sus scrofa
Dynactin subunit 6 Chain: U
Molecule details ›
Chain: U
Length: 190 amino acids
Theoretical weight: 20.7 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: D0G6S1 (Residues: 1-190; Coverage: 100%)
Gene name: DCTN6
Structure domains: Hexapeptide repeat proteins
Dynactin subunit 5 Chain: V
Molecule details ›
Chain: V
Length: 165 amino acids
Theoretical weight: 18.16 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A286ZK88 (Residues: 18-182; Coverage: 91%)
Gene name: DCTN5
Sequence domains: Dynactin subunit 5
Structure domains: Hexapeptide repeat proteins
DYNACTIN SUBUNIT 2 Chain: Y
Molecule details ›
Chain: Y
Length: 243 amino acids
Theoretical weight: 20.7 KDa
Source organism: Sus scrofa
DYNACTIN SUBUNIT 2 Chains: Z, z
Molecule details ›
Chains: Z, z
Length: 419 amino acids
Theoretical weight: 35.68 KDa
Source organism: Sus scrofa
Dynactin subunit 2 Chain: a
Molecule details ›
Chain: a
Length: 48 amino acids
Theoretical weight: 5.4 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A0J9X292 (Residues: 1-48; Coverage: 100%)
Dynactin subunit 2 Chain: b
Molecule details ›
Chain: b
Length: 71 amino acids
Theoretical weight: 7.93 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A0J9X293 (Residues: 1-71; Coverage: 100%)
Sequence domains: Dynamitin
Dynactin subunit 2 Chain: c
Molecule details ›
Chain: c
Length: 31 amino acids
Theoretical weight: 3.17 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A0J9X293 (Residues: 1-20; Coverage: 28%)
Dynactin subunit 2 Chain: d
Molecule details ›
Chain: d
Length: 20 amino acids
Theoretical weight: 2.24 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A0J9X293 (Residues: 1-20; Coverage: 28%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-2856

Quick links

Citations

50 review citations

The cytoplasmic dynein transport machinery and its many cargoes.
Reck-Peterson et al. (2018)
49 more

11 mentions without citation

Roles of actin binding proteins in mammalian oocyte maturation and beyond.
Namgoong et al. (2016)
10 more