› 5aex

X-ray diffraction
3.2Å resolution

Crystal structure of Saccharomyces cerevisiae Mep2

Released:
DOI: 10.2210/pdb5aex/pdb
PDB entry 5aex coloured by chain, front view.
PDB entry 5aex coloured by chain, side view.
PDB entry 5aex coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for Mep2 ammonium transceptor activation by phosphorylation.
van den Berg B, Chembath A, Jefferies D, Basle A, Khalid S, Rutherford JC
Nat Commun 7 11337 (2016)
PMID: 27088325

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154468 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter MEP2 Chains: A, B, C, D, E, F, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, H, I, J
Length: 505 amino acids
Theoretical weight: 54.27 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P41948 (Residues: 1-499; Coverage: 100%)
Gene names: AMT2, MEP2, N1207, N1820, YNL142W
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

1 bound ligand:
Ligand PO4 9 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 103.692Å b: 232.35Å c: 279.189Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.241
Expression system: Saccharomyces cerevisiae

Quick links

Citations

11 review citations

Update on amino acid transporter functions and on possible amino acid sensing mechanisms in plants.
Dinkeloo et al. (2018)
10 more

3 mentions without citation

Structural basis for Mep2 ammonium transceptor activation by phosphorylation.
van den Berg et al. (2016)
2 more