› 5aey

Electron Microscopy
4.3Å resolution

actin-like ParM protein bound to AMPPNP

Released:
DOI: 10.2210/pdb5aey/pdb
PDB entry 5aey coloured by chain, front view.
PDB entry 5aey coloured by chain, side view.
PDB entry 5aey coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles.
Bharat TA, Murshudov GN, Sachse C, Löwe J
Nature 523 106-10 (2015)
PMID: 25915019
Related structures: EMD-2850

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145979 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasmid segregation protein ParM Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 318 amino acids
Theoretical weight: 35.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P11904 (Residues: 1-318; Coverage: 99%)
Gene names: parM, stbA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ANP 5 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-2850
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

A new view into prokaryotic cell biology from electron cryotomography.
Oikonomou et al. (2016)
10 more

5 mentions without citation

Evolution of polymer formation within the actin superfamily.
Stoddard et al. (2017)
4 more