› 5af4

X-ray diffraction
1.85Å resolution

Structure of Lys33-linked diUb

Released:
DOI: 10.2210/pdb5af4/pdb
PDB entry 5af4 coloured by chain, front view.
PDB entry 5af4 coloured by chain, side view.
PDB entry 5af4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Assembly and specific recognition of k29- and k33-linked polyubiquitin.
Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff JL, Freund SM, Komander D
Mol Cell 58 95-109 (2015)
PMID: 25752577

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 76 amino acids
Theoretical weight: 8.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family

Ligands and Environments

2 bound ligands:
Ligand GOL 9 x GOL
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I4
Unit cell:
a: 113.075Å b: 113.075Å c: 103.899Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.231 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Ubiquitin modifications.
Swatek et al. (2016)
23 more

1 mention without citation

Linkage-specific conformational ensembles of non-canonical polyubiquitin chains.
Castañeda et al. (2016)