› 5af5

X-ray diffraction
1.68Å resolution

Structure of Lys33-linked triUb S.G. P 212121

Released:
DOI: 10.2210/pdb5af5/pdb
PDB entry 5af5 coloured by chain, front view.
PDB entry 5af5 coloured by chain, side view.
PDB entry 5af5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Assembly and specific recognition of k29- and k33-linked polyubiquitin.
Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff JL, Freund SM, Komander D
Mol Cell 58 95-109 (2015)
PMID: 25752577

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 73 amino acids
Theoretical weight: 8.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-681; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 28.42Å b: 42.48Å c: 50.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.223
Expression system: Escherichia coli BL21(DE3)

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Citations

24 review citations

Ubiquitin modifications.
Swatek et al. (2016)
23 more