› 5afh

X-ray diffraction
2.4Å resolution

alpha7-AChBP in complex with lobeline

Released:
DOI: 10.2210/pdb5afh/pdb
Model geometry
Fit model/data
PDB entry 5afh coloured by chain, front view.
PDB entry 5afh coloured by chain, side view.
PDB entry 5afh coloured by chain, top view.
Source organisms:
Primary publication:
Molecular blueprint of allosteric binding sites in a homologue of the agonist-binding domain of the α7 nicotinic acetylcholine receptor.
Spurny R, Debaveye S, Farinha A, Veys K, Vos AM, Gossas T, Atack J, Bertrand S, Bertrand D, Danielson UH, Tresadern G, Ulens C
OpenAccess logo Proc Natl Acad Sci U S A 112 E2543-52 (2015)
PMID: 25918415

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153191 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Acetylcholine-binding protein; Neuronal acetylcholine receptor subunit alpha-7 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 205 amino acids
Theoretical weight: 23.7 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P36544 (Residues: 23-39, 52-71, 78-98, 131-137, 139-142, 165-171, 178-192, 198-200, 205-225; Coverage: 24%)
  • Canonical: P58154 (Residues: 36-46, 67-72, 92-122, 130-130, 135-155, 163-168, 187-191, 195-198, 220-224; Coverage: 43%)
Gene names: CHRNA7, NACHRA7
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand L0B 5 x L0B
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 85.52Å b: 112.504Å c: 143.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.168 0.227
Expression system: Spodoptera frugiperda

Quick links

Citations

20 review citations

Biophysics in drug discovery: impact, challenges and opportunities.
Renaud et al. (2016)
19 more

7 mentions without citation

Molecular blueprint of allosteric binding sites in a homologue of the agonist-binding domain of the α7 nicotinic acetylcholine receptor.
Spurny et al. (2015)
6 more