› 5ahk

X-ray diffraction
1.55Å resolution

Crystal structure of acetohydroxy acid synthase Pf5 from Pseudomonas protegens

Released:
DOI: 10.2210/pdb5ahk/pdb
PDB entry 5ahk coloured by chain, front view.
PDB entry 5ahk coloured by chain, side view.
PDB entry 5ahk coloured by chain, top view.
Source organism: Pseudomonas protegens
Entry authors: Dobritzsch D, Loschonsky S, Mueller M, Schneider G

Function and Biology Details

Reaction catalysed: 
2 pyruvate = 2-acetolactate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetolactate synthase II, large subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 588 amino acids
Theoretical weight: 64.93 KDa
Source organism: Pseudomonas protegens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4K6F7 (Residues: 1-568; Coverage: 100%)
Gene names: PFL_5097, ilvB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD

Cofactor: Ligand TPP 2 x TPP
1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 65.22Å b: 139.87Å c: 65.34Å
α: 90° β: 97.87° γ: 90°
R-values:
R R work R free
0.152 0.151 0.174
Expression system: Escherichia coli BL21(DE3)

Quick links