› 5ahn

X-ray diffraction
1.65Å resolution

IMP-bound form of the D199N mutant of IMPDH from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5ahn/pdb
Model geometry
Fit model/data
PDB entry 5ahn coloured by chain, front view.
PDB entry 5ahn coloured by chain, side view.
PDB entry 5ahn coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystallographic studies of two variants of Pseudomonas aeruginosa IMPDH with impaired allosteric regulation.
Labesse G, Alexandre T, Gelin M, Haouz A, Munier-Lehmann H
Acta Crystallogr D Biol Crystallogr 71 1890-9 (2015)
PMID: 26327379

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 489 amino acids
Theoretical weight: 51.77 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HXM5 (Residues: 1-489; Coverage: 100%)
Gene names: PA3770, guaB
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand IMP 1 x IMP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: I4
Unit cell:
a: 116.551Å b: 116.551Å c: 58.247Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The gateway to guanine nucleotides: Allosteric regulation of IMP dehydrogenases.
Buey et al. (2022)
11 other articles