› 5aho

X-ray diffraction
2.16Å resolution

Crystal structure of human 5' exonuclease Apollo

Released:
DOI: 10.2210/pdb5aho/pdb
PDB entry 5aho coloured by chain, front view.
PDB entry 5aho coloured by chain, side view.
PDB entry 5aho coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structures of the SNM1A and SNM1B/Apollo nuclease domains reveal a potential basis for their distinct DNA processing activities.
Allerston CK, Lee SY, Newman JA, Schofield CJ, McHugh PJ, Gileadi O
Nucleic Acids Res 43 11047-60 (2015)
PMID: 26582912

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5' exonuclease Apollo Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 37.9 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9H816 (Residues: 1-335; Coverage: 63%)
Gene names: DCLRE1B, SNM1B
Sequence domains: DNA repair metallo-beta-lactamase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand EDO 6 x EDO
Ligand TLA 2 x TLA
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P4132
Unit cell:
a: 143.092Å b: 143.092Å c: 143.092Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.226
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Involvement of translesion synthesis DNA polymerases in DNA interstrand crosslink repair.
Roy et al. (2016)
3 more

8 mentions without citation

The Chemical Biology of Human Metallo-β-Lactamase Fold Proteins.
Pettinati et al. (2016)
7 more