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› 5aii

X-ray diffraction
1.47Å resolution

Discovery and characterization of thermophilic limonene-1,2-epoxide hydrolases from hot spring metagenomic libraries. CH55-sample-PEG complex

Released:
DOI: 10.2210/pdb5aii/pdb
Model geometry
Fit model/data
PDB entry 5aii coloured by chain, front view.
PDB entry 5aii coloured by chain, side view.
PDB entry 5aii coloured by chain, top view.
Source organism: unidentified
Primary publication:
Discovery and characterization of thermophilic limonene-1,2-epoxide hydrolases from hot spring metagenomic libraries.
Ferrandi EE, Sayer C, Isupov MN, Annovazzi C, Marchesi C, Iacobone G, Peng X, Bonch-Osmolovskaya E, Wohlgemuth R, Littlechild JA, Monti D
FEBS J 282 2879-94 (2015)
PMID: 26032250

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LIMONENE-1,2-EPOXIDE HYDROLASE Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 135 amino acids
Theoretical weight: 14.64 KDa
Source organism: unidentified
Expression system: Escherichia coli
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

8 bound ligands:
Ligand PG4 24 x PG4
Ligand PEG 16 x PEG
Ligand ACT 11 x ACT
Ligand EDO 19 x EDO
Ligand PG5 1 x PG5
Ligand PGE 6 x PGE
Ligand HIS 1 x HIS
Ligand PG6 2 x PG6
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 68.97Å b: 104.23Å c: 148Å
α: 90° β: 102.41° γ: 90°
R-values:
R R work R free
0.177 0.175 0.208
Expression system: Escherichia coli

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Citations

7 review citations

Enzymes from Extreme Environments and Their Industrial Applications.
Littlechild JA. (2015)
6 more

19 mentions without citation

Ocular adhesives: Design, chemistry, crosslinking mechanisms, and applications.
Trujillo-de Santiago et al. (2019)
18 more