› 5ajc

X-ray diffraction
1.7Å resolution

X-ray structure of RSL lectin in complex with sialyl lewis X tetrasaccharide

Released:
DOI: 10.2210/pdb5ajc/pdb
PDB entry 5ajc coloured by chain, front view.
PDB entry 5ajc coloured by chain, side view.
PDB entry 5ajc coloured by chain, top view.
Source organism: Ralstonia solanacearum
Primary publication:
The Hidden Conformation of Lewis x, a Human Histo-Blood Group Antigen, Is a Determinant for Recognition by Pathogen Lectins.
Topin J, Lelimousin M, Arnaud J, Audfray A, Pérez S, Varrot A, Imberty A
ACS Chem Biol 11 2011-20 (2016)
PMID: 27198630

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102700 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Fucose-binding lectin protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 90 amino acids
Theoretical weight: 9.73 KDa
Source organism: Ralstonia solanacearum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0S4TLR1 (Residues: 2-91; Coverage: 99%)
Gene names: E7Z57_08365, HF909_06975, LBW55_09125, RUN39_v1_50103
Sequence domains: Fungal fucose-specific lectin
Structure domains: Lipocalin

Ligands and Environments

Carbohydrate polymer : NEW Components: FUC, NDG, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: FUC, NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: FUC, NDG, GAL, SIA
Carbohydrate polymer
3 bound ligands:
Ligand TRS 1 x TRS
Ligand FUL 3 x FUL
Ligand FUC 2 x FUC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 36.766Å b: 83.163Å c: 90.697Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Predicting the Structures of Glycans, Glycoproteins, and Their Complexes.
Woods RJ. (2018)
5 more

1 mention without citation

Influence of Trp flipping on carbohydrate binding in lectins. An example on Aleuria aurantia lectin AAL.
Houser et al. (2017)