› 5aju

X-ray diffraction
2.5Å resolution

Crystal structure of ligand-free phosphoribohydroxylase lonely guy from Claviceps purpurea in complex with phosphoribose

Released:
DOI: 10.2210/pdb5aju/pdb
PDB entry 5aju coloured by chain, front view.
PDB entry 5aju coloured by chain, side view.
PDB entry 5aju coloured by chain, top view.
Source organism: Claviceps purpurea
Primary publication:
The three-dimensional structure of "Lonely Guy" from Claviceps purpurea provides insights into the phosphoribohydrolase function of Rossmann fold-containing lysine decarboxylase-like proteins.
Dzurová L, Forneris F, Savino S, Galuszka P, Vrabka J, Frébort I
Proteins 83 1539-46 (2015)
PMID: 26010010

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125970 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rossmann fold nucleotide-binding protein Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 29.17 KDa
Source organism: Claviceps purpurea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: M1VUY5 (Residues: 1-240; Coverage: 100%)
Gene name: CPUR_02269
Sequence domains: Possible lysine decarboxylase
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand RP5 1 x RP5
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I4122
Unit cell:
a: 78.97Å b: 78.97Å c: 144.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Biochemical and Structural Aspects of Cytokinin Biosynthesis and Degradation in Bacteria.
Frébortová et al. (2021)
1 more

1 mention without citation

The structure of the Moco carrier protein from Rippkaea orientalis.
Krausze et al. (2020)