› 5al7

X-ray diffraction
2.92Å resolution

N-terminal fragment of Drosophila melanogaster Sas-6 (F143D), dimerised via the coiled-coil domain.

Released:
DOI: 10.2210/pdb5al7/pdb
Model geometry
Fit model/data
PDB entry 5al7 coloured by chain, front view.
PDB entry 5al7 coloured by chain, side view.
PDB entry 5al7 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies.
Cottee MA, Muschalik N, Johnson S, Leveson J, Raff JW, Lea SM
OpenAccess logo Elife 4 e07236 (2015)
PMID: 26002084

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spindle assembly abnormal protein 6 homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 25.17 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli B
UniProt:
  • Canonical: Q9VAC8 (Residues: 2-216; Coverage: 46%)
Gene names: CG15524, SAS6, Sas-6
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NO3 2 x NO3
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P2
Unit cell:
a: 47.13Å b: 64.74Å c: 123.73Å
α: 90° β: 98.91° γ: 90°
R-values:
R R work R free
0.185 0.183 0.215
Expression system: Escherichia coli B

Quick links

Citations

11 review citations

Centrosome function and assembly in animal cells.
Conduit et al. (2015)
10 more

1 mention without citation

The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies.
Cottee et al. (2015)