› 5am9

X-ray diffraction
1.8Å resolution

Crystal structure of the Angiotensin-1 converting enzyme N-domain in complex with amyloid-beta 10-16

Released:
DOI: 10.2210/pdb5am9/pdb
PDB entry 5am9 coloured by chain, front view.
PDB entry 5am9 coloured by chain, side view.
PDB entry 5am9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Kinetic and structural characterization of amyloid-β peptide hydrolysis by human angiotensin-1-converting enzyme.
Larmuth KM, Masuyer G, Douglas RG, Schwager SL, Acharya KR, Sturrock ED
FEBS J 283 1060-76 (2016)
PMID: 26748546

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (6 distinct):
Angiotensin-converting enzyme, soluble form Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 629 amino acids
Theoretical weight: 72.61 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12821 (Residues: 30-658; Coverage: 49%)
Gene names: ACE, DCP, DCP1
Sequence domains: Angiotensin-converting enzyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, BMA
Carbohydrate polymer
10 bound ligands:
Ligand GLN 2 x GLN
Ligand LYS 2 x LYS
Ligand ZN 4 x ZN
Ligand CL 4 x CL
Ligand NA 2 x NA
Ligand PEG 14 x PEG
Ligand P6G 4 x P6G
Ligand GLU 2 x GLU
Ligand VAL 2 x VAL
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 73.348Å b: 101.8Å c: 113.95Å
α: 85.04° β: 85.55° γ: 81.88°
R-values:
R R work R free
0.198 0.197 0.229
Expression system: Cricetulus griseus

Quick links

Citations

2 review citations

ACE2 and ACE: structure-based insights into mechanism, regulation and receptor recognition by SARS-CoV.
Lubbe et al. (2020)
1 more

2 mentions without citation

Kinetic and structural characterization of amyloid-β peptide hydrolysis by human angiotensin-1-converting enzyme.
Larmuth et al. (2016)
1 more