› 5aof

X-ray diffraction
2.45Å resolution

Crystal structure of pneumolysin deletion mutant Delta146_147.

Released:
DOI: 10.2210/pdb5aof/pdb
PDB entry 5aof coloured by chain, front view.
PDB entry 5aof coloured by chain, side view.
PDB entry 5aof coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
CryoEM structures of membrane pore and prepore complex reveal cytolytic mechanism of Pneumolysin.
van Pee K, Neuhaus A, D'Imprima E, Mills DJ, Kühlbrandt W, Yildiz Ö
Elife 6 (2017)
PMID: 28323617

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182321 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pneumolysin Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 54.71 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7ZAK5 (Residues: 1-470; Coverage: 99%)
Gene names: ply, spr1739
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 24.73Å b: 163.5Å c: 207.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.234
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

The gasdermins, a protein family executing cell death and inflammation.
Broz et al. (2020)
16 more

4 mentions without citation

Cholesterol-dependent cytolysins: from water-soluble state to membrane pore.
Christie et al. (2018)
3 more