› 5ap8

X-ray diffraction
2.25Å resolution

Structure of the SAM-dependent rRNA:acp-transferase Tsr3 from S. solfataricus

Released:
DOI: 10.2210/pdb5ap8/pdb
PDB entry 5ap8 coloured by chain, front view.
PDB entry 5ap8 coloured by chain, side view.
PDB entry 5ap8 coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Ribosome biogenesis factor Tsr3 is the aminocarboxypropyl transferase responsible for 18S rRNA hypermodification in yeast and humans.
Meyer B, Wurm JP, Sharma S, Immer C, Pogoryelov D, Kötter P, Lafontaine DL, Wöhnert J, Entian KD
Nucleic Acids Res 44 4304-16 (2016)
PMID: 27084949

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194118 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
16S rRNA aminocarboxypropyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 166 amino acids
Theoretical weight: 19.23 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UWV6 (Residues: 1-166; Coverage: 100%)
Gene names: C21_048, SSO0551
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 49.59Å b: 62.486Å c: 196.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Tuning the ribosome: The influence of rRNA modification on eukaryotic ribosome biogenesis and function.
Sloan et al. (2017)
10 more

1 mention without citation

Ribosome biogenesis factor Tsr3 is the aminocarboxypropyl transferase responsible for 18S rRNA hypermodification in yeast and humans.
Meyer et al. (2016)