› 5apt

X-ray diffraction
1.8Å resolution

Sequence IENKADKAD inserted between GCN4 adaptors - Structure A9

Released:
DOI: 10.2210/pdb5apt/pdb
PDB entry 5apt coloured by chain, front view.
PDB entry 5apt coloured by chain, side view.
PDB entry 5apt coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
α/β coiled coils.
Hartmann MD, Mendler CT, Bassler J, Karamichali I, Ridderbusch O, Lupas AN, Hernandez Alvarez B
Elife 5 (2016)
PMID: 26771248

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 95 amino acids
Theoretical weight: 11.39 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P03069 (Residues: 250-277, 250-281; Coverage: 11%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains: Basic region leucine zipper
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 65.18Å b: 34.59Å c: 67.51Å
α: 90° β: 117.66° γ: 90°
R-values:
R R work R free
0.208 0.206 0.256
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Structure and Topology of α-Helical Coiled Coils.
Lupas et al. (2017)
2 more

1 mention without citation

α/β coiled coils.
Hartmann et al. (2016)