› 5apz

X-ray diffraction
1.6Å resolution

Thermosinus carboxydivorans Nor1 Tcar0761 residues 68-101 and 191-211 fused to GCN4 adaptors

Released:
DOI: 10.2210/pdb5apz/pdb
PDB entry 5apz coloured by chain, front view.
PDB entry 5apz coloured by chain, side view.
PDB entry 5apz coloured by chain, top view.
Source organisms:
Primary publication:
α/β coiled coils.
Hartmann MD, Mendler CT, Bassler J, Karamichali I, Ridderbusch O, Lupas AN, Hernandez Alvarez B
Elife 5 (2016)
PMID: 26771248

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4 Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.93 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: A1HT30 (Residues: 123-211, 226-226; Coverage: 26%)
  • Canonical: P03069 (Residues: 250-263, 265-279; Coverage: 10%)
Gene names: AAS101, AAS3, ARG9, GCN4, TcarDRAFT_0761, YEL009C
Sequence domains: Basic region leucine zipper

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P63
Unit cell:
a: 37.92Å b: 37.92Å c: 179.15Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.177 0.213
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The accommodation index measures the perturbation associated with insertions and deletions in coiled-coils: Application to understand signaling in histidine kinases.
Schmidt et al. (2017)
1 more

2 mentions without citation

The Structure and Topology of α-Helical Coiled Coils.
Lupas et al. (2017)
1 more