› 5aul

X-ray diffraction
1.1Å resolution

PI3K p85 C-terminal SH2 domain/CD28-derived peptide complex

Released:
DOI: 10.2210/pdb5aul/pdb
PDB entry 5aul coloured by chain, front view.
PDB entry 5aul coloured by chain, side view.
PDB entry 5aul coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structures and Thermodynamic Analysis Reveal Distinct Mechanisms of CD28 Phosphopeptide Binding to the Src Homology 2 (SH2) Domains of Three Adaptor Proteins.
Inaba S, Numoto N, Ogawa S, Morii H, Ikura T, Abe R, Ito N, Oda M
J Biol Chem 292 1052-1060 (2017)
PMID: 27927989

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145439 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphatidylinositol 3-kinase regulatory subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27986 (Residues: 614-720; Coverage: 15%)
Gene names: GRB1, PIK3R1
Sequence domains: SH2 domain
Structure domains: SH2 domain
T-cell-specific surface glycoprotein CD28 Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 1.04 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10747 (Residues: 189-196; Coverage: 4%)
Gene name: CD28

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
2 modified residues:
Ligand CSO 1 x CSO
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 40.6Å b: 41.44Å c: 64.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.138 0.16
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Recent Advances in Allogeneic CAR-T Cells.
Kim et al. (2020)
1 more

3 mentions without citation

Crystal Structures and Thermodynamic Analysis Reveal Distinct Mechanisms of CD28 Phosphopeptide Binding to the Src Homology 2 (SH2) Domains of Three Adaptor Proteins.
Inaba et al. (2017)
2 more