› 5avd

X-ray diffraction
0.86Å resolution

The 0.86 angstrom structure of elastase crystallized in high-strength agarose hydrogel

Released:
DOI: 10.2210/pdb5avd/pdb
PDB entry 5avd coloured by chain, front view.
PDB entry 5avd coloured by chain, side view.
PDB entry 5avd coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Growth of protein crystals in hydrogels prevents osmotic shock.
Sugiyama S, Maruyama M, Sazaki G, Hirose M, Adachi H, Takano K, Murakami S, Inoue T, Mori Y, Matsumura H
J Am Chem Soc 134 5786-9 (2012)
PMID: 22435400

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chymotrypsin-like elastase family member 1 Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 25.93 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00772 (Residues: 27-266; Coverage: 96%)
Gene names: CELA1, ELA1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 49.992Å b: 58.21Å c: 74.425Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.128 0.14
Expression system: Escherichia coli

Quick links

Citations

3 review citations

An overview of biological macromolecule crystallization.
Russo Krauss et al. (2013)
2 more

1 mention without citation

The oxygen-oxygen distance of water in crystallographic data sets.
Palese LL. (2020)